Journal: European journal of immunology
Article Title: A human immune system (HIS) mouse model that dissociates roles for mouse and human FcR + cells during antibody-mediated immune responses.
doi: 10.1002/eji.202350454
Figure Lengend Snippet: Figure 2. Human immune cell reconstitution in BRGSA2DR2 Fcer1g−/−mice. Quantification of human immune cell populations in human immune system (HIS) Fcer1g wt mice (black) versus HIS Fcer1g−/−mice (red). (A–C) In blood at 12 weeks postgrafting (wpg), (A) percentage of hCD45 calculated as %hCD45 cells = 100*hCD45/(hCD45 + mCD45), (B) frequency of CD19+ B cells in hCD45 cells, and (C) frequency of CD3+ T cells in hCD45 cells. (D–I) For the spleen, BM and liver at 16 wpg. (D) Absolute numbers of hCD45 per organ (for BM for one femur and one tibia). Frequency of (E) CD19+ B cells, (F) CD3+ T cells, (G) NKp46+ and CD94+ NK cells in hCD45 and (H) CD4+ and (I) CD8+ in total CD3+ T cells. Each dot represents one mouse, and data from the blood are representative for 21 mice (>6 independent experiments) and from the organs for 2 independent experiments. Pairwise comparison in the blood was done by Wilcoxon test, and Kruskal–Wallis test was used for multiple comparisons of means in the organs.
Article Snippet: Antigen Fluorochrome Clone Reactivity Supplier Catalog number CD3 BV510 SK7 Human BioLegend 344828 CD3 APC-Vio770 REA613 Human Miltenyi Biotec 130-113-136 CD4 APC RPA-T4 Human BD 555349 CD4 BUV496 SK3 Human BD 612936 CD11b AF700 M1/70 Mouse/human eBioscience 56-0112-82 CD16 BUV737 3G8 Human BD 564434 CD16 BUV805 3G8 Human BD 748850 CD16/CD32 PE 2.4G2 Mouse BD 561727 CD19 PE HIB19 Human BioLegend 302208 CD19 PE-CF594 HIB19 Human BD 562294 CD20 BV786 2H7 Human BioLegend 302356 CD45 APC-Cy7 30-F11 Mouse BD 557659 CD45 PerCP-Cy5.5 HI30 Human BD 564105 CD45 FITC 30-F11 Mouse BioLegend 103108 CD45 BUV395 HI30 Human BD 563792 CD45 BV650 30-F11 Mouse BD 563410 CD45 BUV805 HI30 Human BD 612891 CD64 PE X54-5/7.1 Mouse BD 558455 CD8a PE-CF594 RPA-T8 Human BD 562282 CD8a BV786 RPA-T8 Human BioLegend 301046 CD8b PE-Cy7 SIDI8BEE Human eBioscience 25-5273-42 CD94 FITC DX22 Human BioLegend 305504 CD94 PerCP-Vio700 REA113 Human Miltenyi Biotec 130-119-763 FCER1A PE MAR-1 Mouse eBioscience 12-5898-82 FCER1G FITC Human/mouse Merck- Millipore FCABS400F Gr-1 FITC RB6-8C5 Mouse BD 553127 NKp46 BV421 9E2/NKp46 Human BD 564065 Antibody-mediated depletion of human cell subsets in vivo For CD8a+ cell depletion, HIS mice were prebled 3 days before i.p. (intraperitoneal) injection with 100 μg/mouse of antihuman CD8a antibody OKT8 (BioXcell) in PBS, and control mice were injected with only PBS and bled at day 4 (d4) postinjection.
Techniques: Comparison
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